Figure 1.

Effect of ulinastatin (UTI) or curcumin (CUR) on cell viability and proliferation of HCT-116. (A) Cytotoxicity of UTI against HCT-116 cells in vitro. Tumor cells (5.0 × 10³) in each well of a 96-well culture plate were incubated for 24 hours at 37°C with or without various concentrations of UTI. CCK-8 was added to each well and, after incubation for 2 hours, the absorbance was measured at 450 nm. (B) HCT-116 cells were treated with different concentrations of CUR for 24 hours, and cell viability was measured using the CCK-8 method. Concentrations of CUR resulting in 50% growth inhibition were indicated as individual IC₅₀ (50% cell growth inhibitory concentrations) values.