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. 2014 Feb 25;5:71. doi: 10.3389/fphys.2014.00071

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Copyright © 2014 Fiori, Reuss, Cuello and Altenberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basic principles and advantages of luminescence resonance energy transfer. The basic gated-mode LRET protocol that takes advantages of the long lifetime of the donor compared to the fluorophore acceptor is summarized at the bottom.