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. 2014 Feb 25;5:22. doi: 10.3389/fendo.2014.00022

Figure 6.

Figure 6

Estradiol-induced actin polymerization requires ROCK and PAK activities. (A,B) Acute hippocampal slices were pre-treated with DMSO (Cnt), H1152 (H11, 200 nM), or PF-3758309 (PF, 50–500 nM) for 30 min and then incubated with estradiol (E2, 10 nM) or BDNF (100 ng/ml) for additional 30 min. At the end of treatments, slices were homogenized and processed for western blot. Data are presented as the ratio (fold of control) of: (A) p-PAK over total PAK3 (N = 3–5), (B) p-cofilin over total cofilin (N = 3–10). (C) Treatments were performed as described above (for E2 the incubation time was 60 min) and slices were fixed, homogenized and labeled with Phalloidin-TRITC (15–30 nM). Data are presented as fluorescence values (fold of control) and are means ± SEM of four to eight independent experiments; **p < 0.01, ***p < 0.001, as compared to control (DMSO); ##p < 0.01, ###p < 0.001, as compared to E2 or BDNF alone (two-way ANOVA).