Figure 8.

Estradiol-induced mTOR-dependent and mTOR–independent protein translation requires actin polymerization. Acute hippocampal slices were pre-treated with DMSO (Cnt) or Latruncullin A (Lat, 0.5–5 μM) for 30 min and then incubated with estradiol (E2, 10 nM) or BDNF (BD, 100 ng/ml) for an additional 30 min. At the end of treatments, slices were homogenized and processed for western blot. Data are presented as the ratio (fold of control) of: (A) p-mTOR over total mTOR (N = 4–5), (B) p-p70S6K (T421/S424) over total p70S6K (N = 4); *p < 0.05, **p < 0.01, as compared to control (DMSO); ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, as compared to E2 or BDNF alone (two-way ANOVA).