Figure 9.

Estradiol-induced mTOR signaling requires PAK activation. (A) Acute hippocampal slices were pre-treated with DMSO (Cnt), H1152 (H11, 200 nM) or PF-03758309 (PF, 50–500 nM) for 30 min and then incubated with estradiol (E2, 10 nM) or BDNF (100 ng/ml) for an additional 30 min. At the end of treatments, slices were homogenized and processed for western blot. Data are presented as the ratio (fold of control) of: (A) p-mTOR over total mTOR (N = 3–13),(B) p-mTOR over total mTOR (N = 3–4); *p < 0.05, **p < 0.01, as compared to control (DMSO); ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, as compared to E2 or BDNF alone (two-way ANOVA).