Table 2.
Effect of combination of carbon and nitrogen sources on xylanase production byAureobasidiumpullulans strain SN090. Fermentation parameters are as follows: pH 4, Incubation time of 48 hr, inoculum size of 2% (v/v), and incubation temperature of 27°C and shaking at 90 rpm
| Organic nitrogen sources | ||||||
|---|---|---|---|---|---|---|
| Yeast Extract | Peptone | |||||
| Carbon sources | Enzyme activity (iu/ml) | Total protein (mg/ml) |
Specific activity (iu/mg) |
Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
| Rice bran | 0.60±0.08 | 0.0117±0.0001 | 51.28 | 0.92±0.26 | 0.0107±0.0002 | 85.98 |
| Wheat bran | 2.21±0.06 | 0.0126±0.0001 | 175.39 | 2.13±0.10 | 0.0126±0.0001 | 169.04 |
| Sugarcane bagasse | 0.91±0.11 | 0.0091±0.008 | 100.00 | 0.51±0.03 | 0.0056±0.0007 | 91.07 |
| Beechwoodxylan | 1.42±0.08 | 0.0091±0.0010 | 156.04 | 1.42±0.03 | 0.0057±0.0003 | 249.12 |
| Casamino acids | Urea | |||||
| Carbon sources | Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
| Rice bran | 1.44±0.20 | 0.0081±0.0009 | 177.77 | 0.26±0.05 | 0.0234±0.0010 | 11.11 |
| Wheat bran | 2.19±0.9 | 0.0126±0.0004 | 173.80 | 1.34±0.02 | 0.0319±0.0001 | 42.00 |
| Sugarcane bagasse | 1.11±0.12 | 0.0047±0.0004 | 236.17 | 0.43±0.07 | 0.0098±0.0004 | 43.87 |
| Beechwoodxylan | 2.66±0.04 | 0.0010±0.0003 | 2660 | 1.57±0.11 | 0.0246±0.0003 | 63.82 |
| Inorganic nitrogen sources | ||||||
| Ammonium nitrate | Ammonium sulfate | |||||
| Carbon sources | Enzyme activity (iu/ml) | Total protein (mg/ml) |
Specific activity (iu/mg) |
Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
| Rice bran | 0.67±0.17 | 0.0070±0.0005 | 95.71 | 0.36±0.02 | 0.0115±0.0001 | 31.30 |
| Wheat bran | 2.38±0.29 | 0.0126±0.0010 | 188.88 | 2.54±0.22 | 0.0152±0.0020 | 167.10 |
| Sugarcane bagasse | 1.56±0.19 | 0.0033±0.0003 | 472.72 | 1.29±0.19 | 0.0029±0.0002 | 444.82 |
| Beechwoodxylan | 2.33±0.09 | 0.0020±0.0008 | 1165 | 2.21±0.025 | 0.0009±0 | 2455.55 |
| Ammonium chloride | Di-ammonium phosphate | |||||
| Carbon sources | Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
Enzyme activity (iu/ml) |
Total protein (mg/ml) |
Specific activity (iu/mg) |
| Rice bran | 0.38±0.04 | 0.0075±0.0007 | 50.66 | 0.44±0.12 | 0.0075±0.0007 | 58.66 |
| Wheat bran | 2.35±0.17 | 0.0129±0.0007 | 182.17 | 2.40±0.21 | 0.0126±0.0001 | 190.47 |
| Sugarcane bagasse | 1.53±0.12 | 0.0008±0.0010 | 1912.50 | 1.30±0.26 | 0.0040±0.0004 | 325 |
| Beechwoodxylan | 2.72±0.15 | 0.0014±0.0003 | 1942.85 | 1.77±0.11 | 0.0014±0.0003 | 1264.28 |
Values are the arithmetic means of results from triplicate experiments