Table 1.
DNA primers for identification of mutations in Tay-Sachs disease
| Exon | Forward | Reverse | Product size (bp) | TM (˚C) |
|---|---|---|---|---|
| E1 | CGTGATTCGCCGATAAGTCA | TCCGACTCACCTGTGAGGTA | 352 | 59.6 |
| E2 | TGTGAGCTGAGGGCTAGAGC | CCAGGCCATCCAGAGTTACA | 250 | 60.0 |
| E3 | CATGAGGTAGGTGGTGCTTTG | TTGCAGTGAGCAGGGACTGG | 453 | 62.0 |
| E4 | GCTACATTGAGAACCTTCCA | ACAGTGATTCCAAACAGA | 313 | 55.6 |
| E5 | TAAGAATCCTGGGAGAGTTG | GGTTACCAGAGTGTCCAGGA | 207 | 57.0 |
| E6 | TGAGAGCTGAGGCAGGTGAA | AACTGGCTGGTTAGGATGAG | 229 | 60.0 |
| E7 | GCATCTTCTACTCTGCTAGC | AAGCTTCACTCTGAGCATAA | 252 | 55.6 |
| E8 | GACACTCATATGGGGTTTTC | GAGTAAGCAACTGATCAGGC | 260 | 55.6 |
| E9 | CAGGCATTAGGCTTTCAGGA | GGCCTGACTCGGTATGGAAA | 223 | 59.6 |
| E10 | CAGTCTAGAACCCATCAGAG | ACTGCTGGTGGCTTCTTCTC | 172 | 59.6 |
| E11 | ACTGCCATTTGACCTTTT | CCATCCTGTGCCCCAACCCA | 267 | 57.0 |
| E12 | GAAACAACTTAGCTGGGGTG | TCCTGCTCTCAGGCCCAAC | 240 | 58.7 |
| E13 | TGTGGATGTCCAGCACCTTT | CTCAGCAACTCACAGCGGAA | 270 | 61.2 |
| E14 | TGACTGGTGTGAAAAGTGTTGCTG | AGGGAGGTGGATGAGTATGC | 690 | 61.2 |