Figure 7.

Protein tyrosine phosphorylation in oocytes from Fyn-null females. (a) Wild-type (WT) and Fyn-null ovaries were probed by western blot using an antibody against Fyn kinase as well as an anti-GADPH antibody as a loading control to confirm that this protein was not present in oocytes or somatic cells. (b) Groups of 20 cumulus-free oocytes from WT and Fyn-null female mice were analysed by western blot using an anti-phosphotyrosine antibody, as well as anti-tubulin as a loading control. Molecular weight standards are presented on the right and arrows indicate the position of phosphorylated tyrosine (P-Tyr)-containing bands that seem to be reduced in Fyn-null oocytes.