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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Stroke. 2013 Jun 6;44(8):2284–2291. doi: 10.1161/STROKEAHA.113.001320

Figure 6.

Figure 6

Increased glycogen-synthase-kinase (GSK)-3β activity impairs mitochondrial transmembrane potential in lipoprotein receptor–related protein-6 (LRP6)+/− mice. A, Representative traces of Ca2+-induced mitochondrial membrane depolarization. Mitochondria isolated from the cerebral cortex of LRP6+/+ or LRP6+/− mice treated with vehicle or the GSK-3β inhibitor SB216763 were exposed to increasing Ca2+ concentrations, and safranine O fluorescence was recorded. Addition of the uncoupling agent carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP; 0.15 μm) to mitochondria from LRP6+/− mice treated with SB216763 led to complete depolarization attesting to the integrity of the preparation. Traces shown are representative of 3 independent experiments with pooled mitochondria from 3 mice in each group. B, Mean fluorescence was recorded during the time period of 500 sec (n=3); *P<0.05 ANOVA followed by the Newman–Keuls test.