Fig. 2. Delay of cell cycle progression in deacetylation inhibition, not in methylation inhibition. (A) Nocodazole treatment induced G2/M phase arrest in A549 cells. TSA treatment increased G2/M phase. However, combination treatment of TSA and nocodazole evidenced a delayed G2/M phase. Each cell population was measured and expressed as a number. (B) AdoX was treated for HMTase inhibition. AdoX had no effect on nocodazole-induced G2/M arrest. (C) Western blot analyses were conducted after TSA treatment in HeLa cells. H3K9ac levels were increased in the TSA treatment group.
