Fig. 3. Increased expression of H3K9me3-responsible HMT-ases. Nocodazole was applied for 12 h, and the at-tached cells and buoyant cells, respectively, were harvested. (A) Western blotting was conducted with various antibodies including SUV39H1, SETDB1, and G9a. Cyclin B was employed as a marker for G2/M phase. (B) Western blot analyses were conducted in order to detect histone modifications. H3K9me3 was increased in the buoyant cells, whereas nei-ther H3K9me1 or H3K9me2 were altered.
