Fig. 7. ATPase activity assays of recombinant ASD and mASD proteins. (A) Purified ASD proteins. Recombinant MBP-ASD and -mASD fusion proteins were prepared in Escherichia coli cells and partially purified. The recombinant proteins were detected by Coomassie staining (left panel) and Western blot analysis using an anti-MBP antibody (right panel). kDa, kilodaltons. (B) ATPase activi-ty assays. ATP hydrolysis activity of recombinant proteins was esti-mated in the presence (+) or absence (-) of 3 mM MgCl2. MBP protein was also included in the assays. Three measurements were averaged (t-test, *P < 0.01). Bars represent standard error of the mean.
