Fig. 8. Effects of abiotic stresses and growth hormones on ASD gene expression. Two-week-old plants grown on MS-agar plates were used for treatments with abiotic stresses and growth hor-mones. Transcript levels were determined by qRT-PCR. Biological triplicates were averaged. Bars indicate standard error of the mean. (A) Effects of abiotic stresses. Plants were exposed to drought, 4℃, or 150 mM NaCl for the indicated time periods before harvesting plant materials for total RNA extraction. (B) Effects of growth hor-mones. Plants were treated with 20 μM ABA, 100 μM SA, or 20 μM MeJA for the indicated time periods before harvesting plant materi-als. (C) Effects of high salinity on ASD gene expression in the aba3-1 mutant.