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. 2012 Mar 17;2(1):2–11. doi: 10.1556/EuJMI.2.2012.1.2

Fig. 8.

Fig. 8.

Molecular intestinal microflora analysis in long-term C. jejuni B2 infected infant mice. Main gut bacterial groups were quantified by molecular analysis of fecal samples before (N, naïve, open circles; n = 10) and after C. jejuni B2 infection of infant mice (d103 p.i., solid circles; n = 10). Quantitative real-time-PCR (polymerase chain reaction) analyses amplified bacterial 16S rRNA variable regions and 16S rRNA gene numbers per nanogram DNA. From feces the following bacterial groups were determined: Enterobacteriaceae (EB), enterococci (EC), lactic acid bacteria (LB), Bifidobacteria (BB), Bacteroides/Prevotella spp. (BP), Clostridium leptum group (CLEP), Clostridium coccoides group (CLOCC), mouse intestinal bacteroidetes (MIB), and total eubacterial load (TL). Numbers of animals harboring the respective bacterial rRNA are given in parentheses. Medians and significance levels (P-values) determined by Mann–Whitney-U test are indicated. Data shown were pooled from three independent experiments.