Table 3.
Pax3-Zic1 targets and their validation by qPCR
| Unigene | Gene
|
Relative expression
|
||||||
|---|---|---|---|---|---|---|---|---|
| Microarray
|
qPCR
|
|||||||
| Symbol | Full name | P | P+Z | Z | P | P+Z | Z | |
| Xl.53491 | hoxd1 | Homeobox D1 | 0.3 | 1.0 | 0.0 | 0.6 | 1.0 | 0.0 |
| Xl.6159 | mfap2 | Microfibrillar-associated protein 2 | 0.4 | 1.0 | 0.0 | 0.9 | 1.0 | 0.1 |
| Xl.20029 | pdgfra | Platelet-derived growth factor receptor alpha | 0.1 | 1.0 | 0.3 | 0.2 | 1.0 | 0.1 |
| Xl.46706 | ak3 | Adenylate kinase 3 | 0.3 | 1.0 | 0.2 | 0.4 | 1.0 | 0.3 |
| Xl.802 | LOC397753 | Hypothetical protein LOC397753 | 0.5 | 1.0 | 0.0 | 1.0 | 1.0 | 0.0 |
| Xl.76016 | fhl3 | Four and a half LIM domain 3 | 0.1 | 1.0 | 0.2 | 1.3 | 1.0 | 0.6 |
| Xl.47341 | ggh | Gamma-glutamyl hydrolase | 0.1 | 1.0 | 0.3 | 0.6 | 1.0 | 0.4 |
| Xl.49589 | abtb2 | Ankyrin repeat and BTB domain containing 2 | 0.1 | 1.0 | 0.4 | 0.4 | 1.0 | 0.8 |
| Xl.14729 | pmp22 | Peripheral myelin protein 22 | 0.1 | 1.0 | 0.3 | 0.2 | 1.0 | 0.5 |
| Xl.14741 | LOC100137640 | Hypothetical protein LOC100137640 | 0.3 | 1.0 | 0.1 | 0.7 | 1.0 | 0.6 |
| Xl.53219 | MGC116527 | Hypothetical protein MGC116527 | 0.3 | 1.0 | 0.3 | n/a | n/a | n/a |
| Xl.76806 | MGC53056 | Similar to selenoprotein T | 0.4 | 1.0 | 0.4 | 1.9 | 1.0 | 1.2 |
| Xl.78260 | slit2 | Slit homolog 2 | 0.3 | 1.0 | 0.4 | 1.1 | 1.0 | 0.4 |
| Xl.40286 | foxa2 | Forkhead box A2 | 0.0 | 1.0 | 0.0 | n/a | n/a | n/a |
| Xl.368 | cer1 | Cerberus 1 | 0.1 | 1.0 | 0.0 | 0.1 | 1.0 | 0.0 |
| Xl.50785 | tfap2e | Transcription factor AP-2 epsilon | 0.4 | 1.0 | 0.1 | n/a | n/a | n/a |
| Xl.1269 | p2ry4 | Pyrimidinergic receptor P2Y, G-protein coupled, 4 | 0.4 | 1.0 | 0.4 | 0.6 | 1.0 | 0.5 |
| Xl.17302 | ppm1k | Protein phosphatase, Mg2+/Mn2+ dependent, 1K | 0.4 | 1.0 | 0.1 | 0.4 | 1.0 | 0.2 |
| Xl.47031 | LOC431899 | Hypothetical protein LOC431899 | 0.4 | 1.0 | 0.1 | 0.8 | 1.0 | 0.1 |
| Xl.689 | nprc | Natriuretic peptide receptor C | 0.2 | 1.0 | 0.1 | 0.3 | 1.0 | 0.2 |
| Xl.697 | nr1i2 | Nuclear receptor subfamily 1, group I, member 2 | 0.2 | 1.0 | 0.0 | 1.2 | 1.0 | 0.0 |
| Xl.1405 | LOC100036942 | Hypothetical protein LOC100036942 | 0.3 | 1.0 | 0.0 | 0.1 | 1.0 | 0.0 |
| Xl.25175 | tspan18 | Tetraspanin 18 | 0.0 | 1.0 | 0.2 | 0.1 | 1.0 | 0.6 |
| Xl.54392 | hoxb4 | Homeobox B4 | 0.2 | 1.0 | 0.0 | n/a | n/a | n/a |
| Xl.47735 | hoxb2 | Homeobox B2 | 0.3 | 1.0 | 0.0 | 1.1 | 1.0 | 0.0 |
| Xl.12464 | lmx1b.1 | LIM homeobox transcription factor 1, beta, gene 1 | 0.4 | 1.0 | 0.2 | 0.8 | 1.0 | 0.3 |
| Xl.30849 | LOC495203 | Hypothetical LOC495203 | 0.1 | 1.0 | 0.2 | 0.2 | 1.0 | 0.8 |
| Xl.67432 | trhd | Tumorhead | 0.2 | 1.0 | 0.2 | 2.6 | 1.0 | 2.0 |
| Xl.17336 | rassf10 | Ras association domain-contain protein 10 | 0.1 | 1.0 | 0.3 | 0.3 | 1.0 | 0.3 |
| Xl.46804 | emp3 | Epithelial membrane protein 3 | 0.2 | 1.0 | 0.1 | 0.2 | 1.0 | 0.2 |
| Xl.34931 | itga5 | Integrin alpha 5 subunit | 0.2 | 1.0 | 0.2 | 0.7 | 1.0 | 0.7 |
| Xl.15219 | b4galt4 | Beta 1,4-galactosyltransferase 4 | 0.3 | 1.0 | 0.2 | 0.7 | 1.0 | 0.2 |
| Xl.23480 | pnhd | Pinhead | 0.3 | 1.0 | 0.4 | n/a | n/a | n/a |
| Xl.47587 | c10orf54 | Chromosome 10 open reading frame 54 | 0.2 | 1.0 | 0.1 | n/a | n/a | n/a |
| Xl.52914 | MGC115333 | Hypothetical protein MGC115333 | 0.3 | 1.0 | 0.4 | n/a | n/a | n/a |
| Xl.50785 | MGC131123 | Hypothetical protein MGC131123 | 0.4 | 1.0 | 0.1 | n/a | n/a | n/a |
P, Pax3-GR; Z, Zic1-GR; n/a, not analyzed. For the qPCR the expression level was normalized to the expression of ef1α.