Fig. 9.
Responses to temperature stress are influenced by knockdown of 5-HT1A receptor in IPCs or globally. Flies with the 5-HT1A receptor knocked down in IPCs by the transgene Dilp2-Gal4/UAS-5-HT 1A-RNAi or globally in the mutant were tested for responses to temperature stress. All experiments were run in two replicates. a, b Response to heat was tested by exposing flies to 39°C and monitoring time to knockdown (given as percent survival with heat). a The flies with the 5-HT1A receptor diminished in IPCs (Dilp2-5-HT1ARi) displayed a faster knockdown at 39°C (p = 0.0005 and p < 0.0001 to parental controls; Log rank test, Mantel-Cox; n= 48–55 for the three genotypes). b The 5-HT1A mutant (mut) flies displayed a similar increased sensitivity to heat compared to wild-type flies (w1118) (p < 0.0001; n = 50 and 54). c, d Recovery from cold knockdown (coma) was monitored in the same genotypes. Flies were kept at 0°C for 4 h and the time to recovery was monitored (given as percent in chill coma). c The flies with receptor knockdown in IPCs were slower in their recovery from cold (p = 0.0009 and p = 0.0017 to controls; n = 52–58 for the three genotypes). d The receptor mutant flies also display a longer recovery time (p < 0.0001 to wild-type control; n = 56 and 58)