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. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: ACS Appl Mater Interfaces. 2013 Sep 27;5(19):9338–9343. doi: 10.1021/am403984k

Figure 7.

Figure 7

Scheme of the TIRF microscope, including acousto-optic modulator (AOM) controlled by frequency generators (Freq. gen.) and electron-multiplying charge coupled device (EMCCD) detector. The top scheme shows the experimental geometry and a cumulative fluorescence image of 1000 frames with the x-dots representing the fitted centers of individual fluorescence binding events on separated frames.