Figure 1.

Schematic representation of preparation of a denaturing gel. (Modified from Sambrook and Russell, Molecular Cloning: A Laboratory Manual 3rd Edition, 2001.) 1: Lay the larger glass plate on the bench and arrange the spacers. 2: Seal three sides of the gel plates with gel-sealing tape. 3: Clamp the plates on both sides with three or four binder clips. 4: Pour the gel solution from a syringe. 5: Insert the comb and cover the top of the gel with plastic wrap.