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Concentrations of polyacrylamide giving optimum resolution of RNA fragments and migration rates of marker dyes using denaturing PAGE (Adapted from Maniatis et al., 1975.)
| Acrylamide (%) | Fragment sizes separated (bases) |
Migration of bromophenol blue (bases) |
Migration of xylene cyanol (bases) |
|---|---|---|---|
| 4 | 100 to 500 | ~50 | ~230 |
| 5 | 70 to 300 | 35 | 130 |
| 6 | 45 to 70 | 26 | 105 |
| 8 | 35 to 45 | 19 | 75 |
| 10 | 25 to 35 | 12 | 55 |
| 20 | 8 to 25 | 8 | 28 |
