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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Stem Cells. 2013 Sep;31(9):1921–1931. doi: 10.1002/stem.1438

Figure 4. Lgr4 loss decreases MRU population and impairs mammosphere formation and self-renewal.

Figure 4

(A) Distribution of Lgr4+/+ and Lgr4−/− mammary epithelial CD45−Ter119−CD31−CD140a− cells by CD24 and CD49f expression. CFC = Colony Forming Cells MYO = myoepithelial cells MRU = Mammary Repopulating Unit. Population gates were set on the basis of comparison with the published populations in Stingl et al. 27.

(B) Subpopulation quantitation (*p = 0.006, n=10).

(C) Mammospheres from Lgr4+/+ and Lgr4−/− MECs over 5 serial passages (**p<0.005; ***p<0.0005; n=5).

(D, E) Photomicrographs of 2nd passage mammospheres (D) and mammosphere size quantification (≥50μm) (E) (***p=0.0002, n=3).