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Morphology and global DNA methylation level of PFFs treated with 5-aza-dC for 72 h. PFF morphology was observed under a light microscope, bar = 100 μm. (A) DNA methylation levels of PFFs treated with different concentrations were determined by flow cytometry. a–c Percentages (% ± SEM) in columns with different superscripts differ significantly (P<0.05).
