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. 2013 Jun 10;59(5):442–449. doi: 10.1262/jrd.2013-026

Fig. 3.

Fig. 3.

Effect of different 5-aza-dC treatment manners on mRNA expression of pluripotent genes during the development of cloned embryos. (A) The relative mRNA expression of Oct4 in porcine embryos derived from IVF, AN, NT and NA was quantified by quantitative real-time PCR. (B) The relative mRNA expression of Nanog in porcine embryos derived from IVF, AN, NT and NA was quantified by quantitative real-time PCR. (C) The relative mRNA expression of Sox2 in porcine embryos derived from IVF, AN, NT and NA was quantified by quantitative real-time PCR. The transcript abundance for every gene at MII was condidered to be the control. The data are expressed as means ± SEM. a–c Percentages at a given stage in columns with different superscripts differ significantly (P<0.05).