Table 3. Development of cloned embryos derived from donor cells treated with 5-aza-dC.
5-aza-dC (nM) | No. (Rep.) | No. embryos fused (% ± SEM) |
No. embryos cleaved (% ± SEM)* |
No. blastocysts (% ± SEM)* |
Total cell numbers of blastocysts (mean ± SEM)& |
0 | 402 (4) | 300 (73.12 ± 4.07)a | 268 (88.19 ± 2.47)a | 64 (20.71 ± 1.55)a | 37 ± 2 (n=63)a |
10 | 341 (4) | 243 (70.65 ± 2.63)ab | 196 (80.98 ± 1.99)b | 44 (18.08 ± 1.04)ab | 34 ± 2 (n=42)ab |
25 | 387 (4) | 228 (58.37 ± 3.80)b | 176 (77.00 ± 2.04)b | 33 (14.23 ± 1.95)b | 34 ± 2 (n=28)ab |
50 | 364 (4) | 207 (56.54 ± 2.88)bc | 161 (77.50 ± 1.90)b | 28 (13.51 ± 1.88)b | 31 ± 2 (n=24)b |
* Cleavage and blastocyst rates were adjusted for fusion rates. & Blastocyst cell numbers of less than 16 were not included. a–c Values in the same column with different superscripts differ significantly (P<0.05).