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. 2013 Jun 10;59(5):442–449. doi: 10.1262/jrd.2013-026

Table 3. Development of cloned embryos derived from donor cells treated with 5-aza-dC.

5-aza-dC (nM) No. (Rep.) No. embryos fused
(% ± SEM)
No. embryos cleaved
(% ± SEM)*
No. blastocysts
(% ± SEM)*
Total cell numbers of blastocysts
(mean ± SEM)&
0 402 (4) 300 (73.12 ± 4.07)a 268 (88.19 ± 2.47)a 64 (20.71 ± 1.55)a 37 ± 2 (n=63)a
10 341 (4) 243 (70.65 ± 2.63)ab 196 (80.98 ± 1.99)b 44 (18.08 ± 1.04)ab 34 ± 2 (n=42)ab
25 387 (4) 228 (58.37 ± 3.80)b 176 (77.00 ± 2.04)b 33 (14.23 ± 1.95)b 34 ± 2 (n=28)ab
50 364 (4) 207 (56.54 ± 2.88)bc 161 (77.50 ± 1.90)b 28 (13.51 ± 1.88)b 31 ± 2 (n=24)b

* Cleavage and blastocyst rates were adjusted for fusion rates. & Blastocyst cell numbers of less than 16 were not included. a–c Values in the same column with different superscripts differ significantly (P<0.05).