Table 4. Development of cloned embryos treated with 5-aza-dC at different concentrations.
| 5-aza-dC (nM) | No. embryos cultured (Rep.) |
No. embryos cleaved (% ± SEM) |
No. blastocysts (% ± SEM) |
Total cell numbers of blastocysts (mean ± SEM) & |
| 0 | 198 (4) | 170 (86.72 ± 3.11) | 40 (19.99 ± 2.16)a | 37 ± 2 (n=37) |
| 10 | 171 (4) | 150 (87.34 ± 2.03) | 37 (21.82 ± 1.19)a | 39 ± 2 (n=35) |
| 25 | 175 (4) | 159 (91.32 ± 2.04) | 50 (28.61 ± 1.44)b | 41 ± 3 (n=49) |
| 50 | 176 (4) | 143 (84.51 ± 2.78) | 41 (23.06 ± 2.57)ab | 37 ± 2 (n=38) |
& Blastocyst cell numbers of less than 16 were not included. a, b Values in the same column with different superscripts differ significantly (P<0.05).