Table 1. Proportions of morphologically normal follicles at the primordial, intermediary, and primary stages after ultrarapid vitrification.
Ovarian sections were vitrified | Primordial follicles | Intermediary follicles | Primary follicles | |||
Mean ± SEM | N | Mean ± SEM | N | Mean ± SEM | N | |
On the Cryosupport | 92.2 ± 2.2a | 239 | 84.5 ± 4.4ab | 126 | 77.2 ± 7.8a | 89 |
On the graphite sheet | 93.3 ± 2.0a | 243 | 81.1 ± 5.4ab | 153 | 87.0 ± 3.7a | 116 |
Between graphite sheets | 84.3 ± 2.8ab | 647 | 65.8 ± 5.7b | 478 | 64.4 ± 14.2a | 138 |
On the copper plate | 70.6 ± 4.3b | 197 | 69.5 ± 6.2ab | 65 | 64.1 ± 16.9a | 34 |
Between copper plates | 36.4 ± 9.0c | 226 | 26.5 ± 11.6c | 208 | 23.6 ± 12.3b | 105 |
Non vitrified | 90.9 ± 3.8a | 417 | 87.9 ± 3.9a | 204 | 70.4 ± 22.6a | 77 |
Four ovarian sections were used to assess the follicle morphology in each experimental condition. Data are shown as the mean ± SEM. N: number of examined follicles. Different superscript letters (a, b, c) in the same row denote significant difference at P<0.05 by ANOVA followed by Fisher’s PLSD test.