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. 2013 Jun 17;59(5):496–499. doi: 10.1262/jrd.2013-012

Table 1. Proportions of morphologically normal follicles at the primordial, intermediary, and primary stages after ultrarapid vitrification.

Ovarian sections were vitrified Primordial follicles Intermediary follicles Primary follicles
Mean ± SEM N Mean ± SEM N Mean ± SEM N
On the Cryosupport 92.2 ± 2.2a 239 84.5 ± 4.4ab 126 77.2 ± 7.8a 89
On the graphite sheet 93.3 ± 2.0a 243 81.1 ± 5.4ab 153 87.0 ± 3.7a 116
Between graphite sheets 84.3 ± 2.8ab 647 65.8 ± 5.7b 478 64.4 ± 14.2a 138
On the copper plate 70.6 ± 4.3b 197 69.5 ± 6.2ab 65 64.1 ± 16.9a 34
Between copper plates 36.4 ± 9.0c 226 26.5 ± 11.6c 208 23.6 ± 12.3b 105
Non vitrified 90.9 ± 3.8a 417 87.9 ± 3.9a 204 70.4 ± 22.6a 77

Four ovarian sections were used to assess the follicle morphology in each experimental condition. Data are shown as the mean ± SEM. N: number of examined follicles. Different superscript letters (a, b, c) in the same row denote significant difference at P<0.05 by ANOVA followed by Fisher’s PLSD test.