Pdyn, Kiss1 and Tac2 mRNA expressions in the ARC
in OVX + low E2 and OVX rats determined by in situ
hybridization. The upper and lower panels show the Pdyn, kiss1 and
Tac2 mRNA expressions in representative rats in the OVX + low
E2 and OVX groups, respectively (A). Insets show the sections at higher
magnification. ARC, arcuate nucleus; ME, median eminence; 3V, third ventricle. The
numbers of Pdyn, Kiss1 or Tac2 mRNA-positive cells
in the ARC of OVX + low E2 and OVX groups (B). Values are means ± SEM.
The numbers in each column represent the numbers of animals used in each group.