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. 2013 Mar 1;59(3):258–265. doi: 10.1262/jrd.2013-001

Fig. 1.

Fig. 1.

Expression profiles of nocturnin in mouse early embryos. A: Quantitative RT-PCR for the expression profile of Nocturnin mRNA in mouse oocytes and early mouse embryos. Different lowercase letters indicate statistical significance (P <0.05). Actin was used as a control. B, C: Immunoblot analysis for the expression profile of Nocturnin protein in mouse oocytes and early embryos. Different lowercase letters indicate statistical significance (P <0.05). Actin was used as a loading control. Moreover, 6xHis-tagged recombinant Nocturnin was used as positive control for Nocturnin antibody. The experiments were performed three times. Different superscripts indicate statistical differences (P <0.05).