Table 2. Primary antibodies for Western blotting.
Protein | Manufacturing company | Molecular weight | Dilution rate |
COX-1 | Cayman Chemical Campany, Ann Arbor, MI, USA; no. 160109 | 70 kDa | 1:200 |
COX-2 | Alpha Diagnostic Intl., San Antonio, TX, USA; no. 70209A | 72 kDa | 1:200 |
PGFS | Received from Dr. K Watanabe | 32 kDa | 1:500 |
PGES | Cayman Chemical; no. 160150 | 23 kDa | 1:500 |
CBR1 | Abcam plc., Cambridge, UK; no. ab4148 | 30 kDa | 1:200 |
Beta-actin | Sigma-Aldrich; no. A2228 | 42 kDa | 1:4000 |