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. 2013 Sep 15;59(6):512–519. doi: 10.1262/jrd.2013-002

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Fig. 3. — Effect of the conceptus-exposed medium (CEM) on TGFβ1 mRNA expression in co-cultured LE and ST cells (A and B) and protein concentration in the incubation medium (C and D). Values from real-time PCR were normalized to GAPDH. Data are expressed as means ± SEM obtained from four experiments (gilts), each performed in duplicate. An asterisk indicates differences between the control and CEM-treated cells (*, P<0.05). A tendency for greater TGFβ1 secretion from LE cells after treatment with CEM is also indicated (#; P=0.07).