Table 2. Fertilization and pronucleus formation in oocytes* reconstructed from brownish (B) and transparent (T) fragments after in vitro fertilization.
Group of oocytes reconstructed | Number of reconstructed oocytes | Number (%) of oocytes penetrated | Number (%) of oocytes with MPN formation |
B oocyte | 72 | 69 (95.8 ± 2.2)a | 65 (94.2 ± 3.8)a |
T oocyte | 24 | 16 (66.7 ± 20.4)b | 8 (50.0 ± 19.1)b |
* Reconstruction of oocytes was carried out with one karyoplast and two cytoplasts for each of the B and T fragments (B and T oocytes). MPN: male pronucleus, defined by the presence of a sperm tail located nearby. In each row, data with different superscripts are significantly different (P<0.05).