Table 4. Genetic cross testing of germline chimeras produced after transfer of DW PGCs with or without freeze-thawing into WT embryos.
Donor PGC treatment/ Chimera ID | Chimera sex | No. of spotted white progeny (donor-derived progeny) | No. of brown progeny(recipient-derived progeny) | Proportion of donor-derived progeny (%) |
Freeze-thaw | ||||
FMQ-1 | Male | 2 | 204 | 1.0 |
FMQ-10 | Male | 0 | 146 | 0 |
FMQ-12 | Male | 4 | 155 | 2.5 |
FMQ-18 | Male | 0 | 128 | 0 |
FMQ-19 | Male | 0 | 113 | 0 |
FMQ-20 | Male | 0 | 6 | 0 |
FMQ-26 | Male | 0 | 3 | 0 |
FFQ-2 | Female | 23 | 21 | 52.3 |
FFQ-7 | Female | 0 | 95 | 0 |
FFQ-8 | Female | 0 | 67 | 0 |
FFQ-14 | Female | 0 | 69 | 0 |
FFQ-15 | Female | 0 | 51 | 0 |
FFQ-21 | Female | 0 | 32 | 0 |
FFQ-25 | Female | 0 | 82 | 0 |
Mean ± SE | 4.0 ± 3.7 | |||
Unfrozen controls | ||||
UMQ-3 | Male | 69 | 80 | 46.3 |
UMQ-7 | Male | 18 | 99 | 15.4 |
UMQ-10 | Male | 34 | 141 | 19.4 |
UMQ-13 | Male | 36 | 98 | 26.9 |
UMQ-18 | Male | 2 | 86 | 2.3 |
UFQ-1 | Female | 9 | 44 | 17.0 |
UFQ-2 | Female | 3 | 76 | 3.9 |
UFQ-11 | Female | 8 | 80 | 9.1 |
UFQ-15 | Female | 0 | 61 | 0 |
UFQ-17 | Female | 0 | 89 | 0 |
Mean ± SE | 14.0 ± 4.6 |