Table 1. SAIS analysis of thawed semen from the extinct JBC elite bull (BK94-13) and post-death cloned bull (Heuk Oll Dolee)*.
Parameter** | Unit | Result | Reference | |
BK94-13 | Heuk Oll Dolee | |||
No. of fields analyzed | Fields | 5 | 5 | |
No. of sperm analyzed | Cells | 410.2 | 450.8 | >>100 |
Concentration | Millions | 27.2 | 29.8 | |
Total concentration | Millions/ml | 135.8 | 149.2 | >>20 |
Motility | (%) | 90.7 | 93.3 | >>50 |
VCL (curvilinear velocity) | µm/sec | 183.3 | 188.6 | >>46 |
VSL (straight-line velocity) | µm/sec | 41.4 | 48.2 | >>26 |
VAP (average path velocity) | µm/sec | 88.7 | 97.1 | |
Lin (linearity) | 24.1 | 26.7 | >>58 | |
ALH (amplitude of lateral head displacement) | µm | 4.3 | 4.3 | >>2.4 |
STR (straightness) | 46.4 | 49.2 | ||
BCF (beat-cross frequency) | Hz | 9.3 | 9.7 | |
MAD (mean angular displacement) | Degrees | 54.6 | 57.3 | |
WOB (wobble) | 48.3 | 51.4 |
* The experiment was replicated three times. ** Semen collection time: BK 94-13 (at 13 years of age) and Heuk Oll Dolee (at 25 months of age).