Figure 5. P- and E-selectin interactions are required for memory CD8⁺ T cell recruitment to inflamed tissues.

(A) 1 × 10⁶ purified memory P14 CD8⁺ T cells (Thy1.1) were transferred into naive B6 mice (Thy1.2) that were then challenged intranasally with either saline or CpG. Three days after challenge, memory P14 CD8⁺ T cells in the lung were analyzed for coexpression of core 2 O-linked glycosylated CD43 (1B11) and the ability to bind to either P- or E-selectin. (B and C) 1 × 10⁶ memory P14 CD8⁺ T cells were transferred into naive B6 mice followed by intranasal challenge with either saline or CpG. On day 3 after challenge, total memory P14 CD8⁺ T cells in the lungs were quantified in mice that received i.v. administration of an isotype control antibody or blocking antibodies against either (B) P- and E-selectin or (C) PSGL-1. (D) Mice containing memory P14 CD8⁺ T cells were generated as in Figure 3 and infected on the left ear with VacV-GP₃₃. Total P14 CD8⁺ T cells in the ears were quantified on day 3 after infection in mice receiving an isotype control or P- and E-selectin–blocking antibodies. (E) Same as in D, with the exception that viral burden of the ear was determined on day 4 after infection. Data are representative of 2 or more independent experiments, and statistical analysis used 1-way ANOVA with a Bonferroni post-test for multiple comparisons.