Figure 6. Inflammation is sufficient to generate core 2 O-glycans on circulating memory CD8⁺ T cells.

(A) Pre- and post-sort analysis following depletion of memory P14 CD8⁺ T cells isolated from the spleen that expressed core 2 O-linked glycosylated CD43 (1B11). (B) 1 × 10⁶ 1B11^lo cells (Thy1.1) from A were transferred into naive B6 mice (Thy1.2) that were then challenged with either saline or CpG. Three days after challenge, memory P14 CD8⁺ T cells in the lungs were analyzed for the expression of core 2 O-linked glycosylated CD43 (1B11). (C and D) 1 × 10⁶ purified memory P14 CD8⁺ T cells (Thy1.1) were transferred into naive B6 mice (Thy1.2) followed by intranasal challenge with either saline or CpG. One group of mice received pertussis toxin (Ptx) following CpG challenge. On day 3 after challenge, the number of memory P14 CD8⁺ T cells in the (C) lung and (D) blood was determined. (E) Expression of core 2 O-linked glycosylated CD43 on memory P14 CD8⁺ T cells in the lung and blood from C and D. Data are representative of 2 independent experiments, and statistical analysis used 1-way ANOVA with a Bonferroni post-test for multiple comparisons.