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. 2014 Feb 3;124(3):1309–1319. doi: 10.1172/JCI67947

Figure 2. Decreased DC number in the epidermis and dermis of HYAL1-overexpressing mice.

Figure 2

(A) Representative dot plots of MHC class II and CD11c double-positive viable DCs from epidermal sheets of transgenic mice (control, CAG-GFP mice). Numbers within plots denote the percentage of cells within the respective gates. IA/IE, MHC class II. (B) Percentage of MHC class II+CD11c+ DCs in epidermal sheets of mice by flow cytometry (***P < 0.001; n = 3). (C) Immunohistochemistry of MHC class II in epidermal sheets. Scale bar: 50 μm; n = 6. (D) Dermal single cell suspensions of K14/HYAL1 mice and control (K14-Cre) mice were subjected to flow cytometry analysis, and MHC class II– and CD11c-positive cell numbers were assessed (*P < 0.05; n = 3). Mean ± SEM. (E) Number of langerin-positive cells among MHC class II– and CD11c-positive cells (*P < 0.05; n = 3). Mean ± SEM. (F) Flow cytometry analysis of dermal single cell suspensions showing expression of CD11b and CD103 from K14/HYAL1 and control (K14-Cre) mice. Cells were gated on langerin+, MHC class II+, and CD11c+ cells. (G) Cells gated on langerin-negative, MHC class II+, and CD11c+ cells showing expression of CD11b and CD103. Numbers represent the percentage of the cells in the indicated gate. Data are representative of 3 independent experiments.