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. 2014 Feb 10;124(3):1296–1308. doi: 10.1172/JCI72051

Figure 4. AXL and LRP-1 cooperate to facilitate DC efferocytosis.

Figure 4

(A and B) In vivo splenic DC efferocytosis was assayed in the indicated mice (n = 3 per group) 3 hours after injection of PKH67-labeled ACs. TIM-3 nAb (100 μg) was administered i.p., and GST-RAP (2 mg/mouse) was administered i.v., 30 minutes before AC injection. (C) BMDCs derived from WT or Axl–/– mice were treated with 5 μg/ml cytochalasin D to block AC internalization. To measure AC binding, cells were incubated with fluorescently labeled ACs for 1 hour in the absence or presence of 1 μg/ml GST-RAP to block LRP-1. (D) Similar to C, except the experiment was conducted in the absence of cytochalasin D to measure AC engulfment. n = 3 independent experiments. *P < 0.05 vs. respective control; #P < 0.05 vs. all other groups.