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. 2014 Feb 24;124(3):1406–1417. doi: 10.1172/JCI70454

Figure 4. BRAFi induces an early ER stress response.

Figure 4

(A) Immunoblotting against the indicated signaling markers after treatment of MEL624 cells with vehicle or 1 μM PLX4720 for the indicated times. P-, phospho-. (B) MEL624 and A375P cells were treated with 1 μM PLX4720 for the indicated times. Whole-cell and nuclear lysates were subjected to immunoblotting. Tg, thapsigargin. (C) PLA performed on MEL624 cells treated for 30 minutes with DMSO or 10 μM PLX4720 and immunofluorescence for the ER resident protein disulfide isomerase (green), which reflects total ER area. Red fluorescence reflects a protein-protein interaction. Original magnification, ×40. Quantification of red, green, and yellow (colocalization) signals (mean ± SD) reflects at least 3 separate experiments.