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LC-CoA binding to NHE1 was determined by SPR, using immobilized NHE1 cytosolic domain (cNHE1) peptide as ligand and LC-CoAs as analytes, as described in Methods. (A) SPR sensorgrams for palmitoyl-CoA (Kd = 3.4 ± 0.9 × 10–5 M; n = 3). (B) SPR sensorgrams for oleoyl-CoA (Kd = 2.3 ± 0.3 × 10–5 M; n = 3). (C) SPR sensorgrams for palmitoyl-CoA at a range of pH values.
