Figure 5. mTregs in human skin are nonmigratory.

(A) CCR7 expression on mTregs and mTconvs in human skin harvested from healthy adults. Cells were pregated on viable CD3⁺CD4⁺CD45RO⁺ cells. (B) Human skin was grafted onto immunodeficient NSG mice, and at specific times thereafter, grafted skin, blood, and spleen (not shown) were harvested and human T cells analyzed by flow cytometry. Cells were pregated on viable CD3⁺CD4⁺hCD45⁺ cells. (C) Absolute numbers of CD3⁺CD4⁺hCD45⁺Foxp3⁺ and CD3⁺CD4⁺hCD45⁺Foxp3^– cells in grafted skin and blood 3 and 7 weeks after grafting. Results are pooled data from two of five representative experiments with two or more mice per group. P values were determined using a 2-tailed unpaired Student’s t test.