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. 2014 Feb 17;124(3):1173–1186. doi: 10.1172/JCI72230

Figure 2. Upregulation of CASP6 in the spinal cord and CSF after acute inflammation.

Figure 2

(A) Double staining of CASP6 and CGRP in the ipsilateral and contralateral dorsal horn 30 minutes after formalin injection. Scale bar: 100 μm. (B) Triple staining of CASP6, CGRP, and CX3CR1 (GFP) in the ipsilateral dorsal horn 30 minutes after formalin injection. Note close contacts between CASP/CGRP-expressing axonal terminals and microglial cell body and processes. Scale bar: 10 μm. (C and D) Western blot analysis showing the bands of active CASP6 (aCASP6, ≈20 and 10 kDa) 30 minutes after the formalin (5%) injection and 15 minutes after bradykinin (300 ng) injection. (D) Intensity of aCASP6 bands. *P < 0.05, n = 3–4 mice. (E and F) Western blotting analysis showing increase of aCASP6 in the CSF. The positive control band of rCASP6 is indicated. (F) Intensity of aCASP6 bands. *P < 0.05, n = 5 rats.