Skip to main content
. 2014 Feb 17;124(3):1173–1186. doi: 10.1172/JCI72230

Figure 7. Intrathecal injection of rCASP6 induces mechanical allodynia via microglial and TNF-α signaling.

Figure 7

(A) Intrathecal rCASP6 but not rCASP3 (5 U) elicits persistent mechanical allodynia. *P < 0.05 versus vehicle (PBS), n = 6–8 mice. (B) rCASP6-induced (i.t., 5 U) mechanical allodynia is abrogated in Tnfr double knockout (Tnfr1/2 DKO) mice. *P < 0.05, n = 7 mice. (C) rCASP6 (i.t., 5 U, 3 hours) increases TNF-α levels in spinal cord but not DRG tissues. *P < 0.05, n = 4 mice. (D) rCASP6-induced (i.t., 5 U) mechanical allodynia is reduced by minocycline pretreatment (i.t., 50 μg). *P < 0.05, n = 5 mice. (E) Spinal (i.t.) injection of rCASP6-stimulated microglia, but not control microglia, induces mechanical allodynia. *P < 0.05, n = 5–7 mice. (F) Transient reversal of mechanical allodynia following injection of rCASP6-stimulated microglia by i.t. TNF-α–neutralizing antibody (5 μg). *P < 0.05, n = 5–7 mice.