(A) Sagittal sections were labeled for CD11c by IHC, followed by IF staining for DCX (green), showing the course of the RMS from the subventricular zone to the OB. AOV, ventral anterior olfactory nucleus; ACB, nucleus accumbens; CP, caudate putamen. Box indicates the region shown at higher magnification in B. (B) The RMS, showing CD11c cells (red arrowheads) in close association with migrating DCX+ neuroblasts. (C) CD11c+ cells in the RMS were counted in at least 5 sections. The corpus callosum (CC) and frontal cortex (FCx) in the same sections were similarly examined. Data represent mean ± SEM. *P < 0.001 vs. RMS. (D) Phenotypic analysis of CD11c+ cells isolated from 10 mouse forebrains (for gating strategy, see Supplemental Figure 2). CD11c+CD11b+ cells represented 70% of DCs, pDCs represented 10%, and the remainder belonged to other CD11c+CD11b– DC subsets (3 independent experiments). (E) IF confocal images for DCX (green), CD11b (blue), and CD11c (red). CD11c+ (red arrowhead) and CD11b+ (blue arrowhead) cells are identified in the choroid plexus adjacent to the DCX+ subventricular zone in normal mice. (F) CD11c+CD11b+ DCs (blue arrow), CD11c+CD11b– DCs (red arrow), and CD11b+ microglia (white arrows) in close association with the RMS (green; DCX). Scale bars: 250 μm (A); 25 μm (B); 10 μm (E and F).