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. 2014 Feb 3;124(3):1144–1157. doi: 10.1172/JCI71919

Figure 4. Dynamics of GFP-labeled desmin and α-actinin in differentiated myotubes monitored by FRAP analyses.

Figure 4

(A and B) Representative (A) desmin-GFP– or (B) α-actinin–GFP–expressing Plec+/+ and Plec–/– myotubes (differentiation for 10 days) are shown prior to photobleaching, immediately after photobleaching, and after 10 and 28 minutes of recovery. Bleached areas are indicated by boxes. Scale bars: 10 μm. (C) FRAP analyses of desmin-GFP in Plec+/+ and Plec–/– myotubes differentiated for 5 or 10 days. Mean ± SEM, 2 experiments (5 days of differentiation: Plec+/+, n = 25 analyzed ROIs; Plec–/–, n = 21 analyzed ROIs; 10 days of differentiation: Plec+/+, n = 54 analyzed ROIs; Plec–/–, n = 55 analyzed ROIs). (D) FRAP analysis of α-actinin–GFP under conditions similar to those in C. Mean ± SEM, 2 experiments (5 days of differentiation: Plec+/+, n = 25 analyzed ROIs; Plec–/–, n = 26 analyzed ROIs; 10 days of differentiation: Plec+/+, n = 38 analyzed ROIs; Plec–/–, n = 45 analyzed ROIs).

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