Figure 5. Expression profiles of chaperones in plectin-deficient muscle fibers and differentiated myotubes.

(A) Equal amounts of wild-type and plectin-deficient (MCK-Cre/cKO) gastrocnemius muscle lysates were subjected to immunoblotting analysis using antibodies as indicated. GAPDH was used as loading control. (B) Signal intensities were densitometrically measured and normalized to total protein content. Mean ± SEM, 3 experiments. (C) Plec^+/+ and Plec^–/– myoblasts were differentiated for the time periods indicated, and cell lysates prepared at days 0, 5, 10, and 15 were analyzed by immunoblotting as in A. (D) Signal intensities of HSP27 protein bands shown in C were measured and normalized as in B. Mean ± SEM, 3 experiments. *P < 0.05, **P < 0.01, unpaired Student’s t test.