Figure 3. Absence of CXCL5 or its receptor CXCR2 protects from TB-associated wasting disease and death.

(A) Kaplan-Meier curves showing survival of Cxcr2^+/+ (n = 27) and Cxcr2^–/– (n = 20) mice after M. tuberculosis infection (∼570 CFUs). Results are pooled from 2 independent experiments (log-rank test). (B) Kaplan-Meier curves showing survival of WT (n = 41) and Cxcl5^–/– (n = 36) mice after M. tuberculosis infection (∼500 CFUs). Results are pooled from 4 independent experiments (log-rank test). (C) Relative weight curves of Cxcr2^+/+ and Cxcr2^–/– mice during M. tuberculosis infection (∼570 CFUs, n ≥ 20 [n_{WT day 28 p.i.} = 9]). Data are pooled from 2 independent experiments. (D) Relative weight curves of WT and Cxcl5^–/– mice during M. tuberculosis infection (∼500 CFUs; n ≥ 36 [n_{WT day 28 p.i.} = 35]). Data are pooled from 4 independent experiments. (E) Pulmonary M. tuberculosis burden in WT and Cxcl5^–/– mice (∼500 CFUs; n_pooled = 9–10 per time point). Data are pooled from 5 independent experiments. (C–E) Mean ± SEM, 2-way ANOVA/Bonferroni post-test. (F) Proinflammatory cytokine levels in lung homogenates of WT and Cxcl5^–/– mice at day 30 p.i. (mean ± SEM; n_pooled = 10). Data are pooled from 2 independent experiments (unpaired t test). (G) Lung histopathology at 21 days p.i. as shown by hematoxylin and eosin staining. Scale bar: 1,000 μm (top row); 100 μm (bottom row). Data are representative of 3 independent experiments (n = 3–5). *P < 0.05; **P < 0.01; ****P < 0.0001.