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. 2014 Feb 10;124(3):1268–1282. doi: 10.1172/JCI72030

Figure 9. CXCL5 expression in epithelial cells is induced by M. tuberculosis TLR2 agonists.

Figure 9

(A) CXCL5 abundance measured by ELISA in supernatants of the AECII cell line (T7) stimulated with M. tuberculosis components and TLR agonists: γ-irradiated M. tuberculosis (Irr Mtb; MOI 10 and 100), 10 μg/ml cell wall (CW), 10 μg/ml membrane (MEM), 10 μg/ml cytosolic fraction (CytF), 10 μg/ml total lipids (TLIPs), 5 μg/ml muramyl dipeptide (MDP), 5 μg/ml mycolylarabinogalactan-peptidoglycan (mAGP), 5 μg/ml trehalose-6,6′-dimycolate (TDM), 5 μg/ml peptidoglycan (PG), 5 μg/ml mannose-capped lipoarabinomannan (ManLAM), 5 μg/ml phosphatidyl-myo-inositol mannosides 1 and 2 (PIMs), 5 μg/ml lipoarabinomannan (LAM), 1 μg/ml lipomannan (LM), 1 μg/ml 19-kDa lipopeptide (19kDa LP), 1 μg/ml 27-kDa lipopeptide (27kDa LP), 1 μg/ml MPT83 lipopeptide (MPT83 LP), 10 μg/ml zymosan (Zyn), 1 μM CpG, 1 μg/ml PAM3CSK4, 1 μg/ml LPS (1-way ANOVA/Bonferroni post-test). (B and C) CXCL5 concentration measured by ELISA in supernatants of AECII (T7) treated with and without anti-TLR2 mAb and (B) stimulated for 24 hours with γ-irradiated M. tuberculosis, 19-kDa and 27-kDa lipopeptides, MPT83, PAM3SCK4, or LPS and (C) infected with M. tuberculosis (Mtb) (2-way ANOVA/Bonferroni post-test). (A–C) Data are representative of 2 independent experiments (mean ± SEM; n = 3). (D) Chemokine measurements by ELISA and (E) PMN numbers and frequencies determined by flow cytometry in BAL fluid of high-dose (∼400 CFUs) M. tuberculosis–infected WT and Tlr2–/– mice (mean ± SEM; npooled = 10). Data are pooled from 2 independent experiments (2-way ANOVA/Bonferroni post-test). **P < 0.01; ***P < 0.001; ****P < 0.0001.