Table 1. Description of forward (FP) and reverse (RP) primers used to assess expression of target genes in blastocysts acquired from different culture conditions.
Genes | Sequence (5´-3´ orientation) | Fragment length (bp) | GenBank accession number or Reference |
HSP701 | FP: ATCCAGGTGTACGAGGG RP: TGGTGATCTTGTTGGCCT |
190 | L16764 |
BAX | FP: CCGATGGCAACTTCAACTGGG RP: GTCAGCACTCCCGCCACAAAG |
244 | [35] |
BCL-2 | FP: GGAGGATTGTGGCCTTCT RP: GTTATCCTGGATCCAGGTGT |
143 | [36] |
GAPDH | FP: GGAGAAAGCTGCCAAATATG RP: CAGGAAATGAGCTTGACAAAGTGG |
191 | [28] |
1HSP70 primers were used for feline fibroblast (experiment 1) and blastocysts (experiment 2).