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. 2013 Jan 25;59(2):180–188. doi: 10.1262/jrd.2012-116

Table 1. Description of forward (FP) and reverse (RP) primers used to assess expression of target genes in blastocysts acquired from different culture conditions.

Genes Sequence (5´-3´ orientation) Fragment length (bp) GenBank accession
number or Reference
HSP701 FP: ATCCAGGTGTACGAGGG
RP: TGGTGATCTTGTTGGCCT
190 L16764
BAX FP: CCGATGGCAACTTCAACTGGG
RP: GTCAGCACTCCCGCCACAAAG
244 [35]
BCL-2 FP: GGAGGATTGTGGCCTTCT
RP: GTTATCCTGGATCCAGGTGT
143 [36]
GAPDH FP: GGAGAAAGCTGCCAAATATG
RP: CAGGAAATGAGCTTGACAAAGTGG
191 [28]

1HSP70 primers were used for feline fibroblast (experiment 1) and blastocysts (experiment 2).