. 2013 Jan 25;59(2):180–188. doi: 10.1262/jrd.2012-116

Table 3. Mean percent ± SE of developmental competence of cat embryos (3 replicates) cultured in groups of ten in different volume of medium to acquire different culture densities.

Culture volume (µl)	Culture density	N	Cleaved embryos developed to		Hatching	Cell no.	Fragmentation
Culture volume (µl)	Culture density	N	Morula	Blastocyst	Hatching	Cell no.	Fragmentation
12.5	1:1.25	50	81.7 ± 7.3^a	15.0 ± 10.4^a	0.0 ± 0.0^a	69.1 ± 42.7^a	19.5 ± 12.1^a
25	1:2.5	50	80.0 ± 7.6^a	20.0 ± 5.0^ab	13.3 ± 13.3^a	180.1 ± 41.8^b	22.5 ± 6.6^a
50	1:5	60	97.2 ± 1.5^a	39.4 ± 7.5^bc	21.0 ± 1.0^a	140.5 ± 15.4^ab	14.3 ± 1.4^a
100	1:10	50	86.7 ± 3.3^a	48.3 ± 1.7^c	14.1 ± 7.1^a	149.2 ± 14.9^ab	14.4 ± 1.5^a
200	1:20	60	90.6 ± 4.7^a	43.3 ± 3.3^c	19.4 ± 10.0^a	152.2 ± 28.0^ab	14.3 ± 1.2^a

^{a, b, c} Within a column, values with different superscripts were significantly different (P<0.05). N = Total number of cleaved embryos used. Culture density = 10 embryos: medium volume (µl).