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. 2012 Dec 20;59(2):151–158. doi: 10.1262/jrd.2012-122

Table 1. Primers used for RT-PCR.

Genes	Primer sequences (5´– 3´)^a	Annealing temperature (C)	Fragment size (bp)	GenBank accession no.
OCT-4	F- GTTCTCTTTGGGAAGGTGTT	56	313	AJ251914
OCT-4	R- ACACGCCGGACCACATCCTTC	56	313	AJ251914
CDX2	F- GTCACCAGAGCTTCTCTGGG	53	144	EU137688
CDX2	R- AGACCAACAACCCAAACAGC	53	144	EU137688
TEAD4	F- AAGTTCTGGGCAGACCTCAA	60	157	XM_605145
TEAD4	R- GTGCTTCAGCTTGTGGATGA	60	157	XM_605145
GATA3	F- CATGTCCTCTCTCAGCCACA	60	206	NM_001044567
GATA3	R- TGCGAAAATGCACGTAGAAG	60	206	NM_001044567
NANOG	F- GTACCTCAGCCTCCAGCA	57	161	AJ877915
NANOG	R- CTGAGCCCTTCTGAATCAC	57	161	AJ877915
FGF4	F- TTCTTCGTGGCCATGAGCAG	52	206	XM_003122418
FGF4	R- AGGAAGTGGGTGACCTTCAT	52	206	XM_003122418
FGFR1-IIIc	F- ACTGCTGGAGTTAATACCACCG	60	125	AJ577088
FGFR1-IIIc	R- GCAGAGTGATGGGAGAGTCC	60	125	AJ577088
FGFR2-IIIc	F- GGTGTTAACACCACGGACAA	60	139	AJ439896
FGFR2-IIIc	R- CTGGCAGAACTGTCAACCAT	60	139	AJ439896
GAPDH	F- TCGGAGTGAACGGATTTG	60	219	AF017079
GAPDH	R- CCTGGAAGATGGTGATGG	60	219	AF017079

^a Primer orientations: F, forward; R, reverse.