Table 4. Effect of embryo density on the number of cells in blastocysts in droplet or WOW cultures*.
Culture system | Embryos/dish | Total embryos examined |
Number of cells/blastocyst a | ICM% (ICM/total)a | ||
Total | ICM | TE | ||||
Droplet | 5 | 19 | 97.7 ± 53.6 | 32.5 ± 18.9 | 65.3 ± 37.2 | 34.0 ± 9.5 |
15 | 25 | 91.3 ± 40.0 | 36.4 ± 15.3 | 54.8 ± 26.9 | 40.8 ± 8.2 | |
25 | 33 | 107.1 ± 37.0 | 39.4 ± 17.9 | 67.7 ± 29.2 | 37.5 ± 13.1 | |
WOW | 5 | 19 | 109.2 ± 33.2 | 36.4 ± 13.3 | 72.8 ± 27.4 | 33.8 ± 9.9 |
15 | 30 | 122.1 ± 37.8 | 46.1 ± 18.3 | 75.9 ± 25.3 | 37.5 ± 7.7 | |
25 | 43 | 107.1 ± 38.2 | 37.2 ± 20.6 | 70.8 ± 25.7 | 33.1 ± 12.2 |
* Values were created with embryos from 5 replicate trials. a Values indicate the mean ± SD. ICM, inner cell mass; TE, trophectoderm.